A solution of BSA is made up by weighing out 20 mg of the

BSA made up weighing out mg the lyophilized (freeze dried) powder the protein supplied dissolving mL water. The concentration the protein was checked adding mL the solution to mL water; the A280 the resulting solution relative to a water blank was . The absorption coefficient BSA (mg mL cm”‘. Calculate the actual concentration of the original solution of BSA and comment on your answer.